Algae and Cyanobacteria from Extreme Environments

The research group focusing on algae and cyanobacteria from extreme environments of polar and mountain regions promote their findings about the algae they isolated as highly interesting for applications in biotechnological production of high-value chemicals, such as carotenoids, polyunsaturated fatty acids and phosphatidyl glycerol used mainly as nutraceuticals, pharmaceuticals, in cosmetics etc. The main objectives of researchers are the description of unknown biodiversity, ecology and ecophysiology, biogeography and investigation of the mechanisms of adaption of algae on the extreme conditions they thrive in. Last but not least, is the role of algae as important bioindicators for water quality assessment over long time periods, moreover for evaluating the whole ecosystem recovery from acid rains or the climate changes in the past. The well-established isolation techniques and cultivation methods serve for successful maintaining extremophilic algae and cyanobacteria in laboratory conditions and for further optimization of their growth for scaled-up biotechnological productions. One particular algae strain has already become a part of a patent.

For whole presentation including contact details see this document.

Clinical Proteomics LAB

We offer:

  • Identification of proteins from gels and solutions (MS and MS / MS).

  • Proteomic analyzes of complex samples.

  • Marking and quantitative proteomic analysis of iTRAQ, SILAC etc.

  • Proteomic analysis of cells and tissues of all types using iTRAQ, TMT or SILAC isotope methods.

  • Proteomic analysis of integral membrane proteins.

  • Training and service in the fi eld of two-dimensional electrophoresis and other proteomics techniques.

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CLIP Genomics

We offer:

  • NGS data analysis service: We off er bioinformatic analysis of next generation sequencing data from all current NGS platforms. From your primary data we will create well-ordered and easily accessible results. Our working group has an adequate hardware for the analysis of large-scale NGS data. Our specialists have both mathematical and biological education which ensures a comprehensive approach to the results. Amplicon/exome sequencing – variant analysis - DNA sequencing of selected parts of the genome (amplicons) or all exons of protein-coding genes. Transcriptome sequencing (mRNA-Seq) - mRNA-sequencing for gene expression analysis. Methylome sequencing (RRBS) - sequencing of GpG-rich areas and their broader neighbourhood for the assessment of DNA methylation. Chromatin immunoprecipitation sequencing (ChIP-Seq) - sequencing of DNA after chromatin immunoprecipitation for the DNA-protein analysis.

  • NGS data analysis courses: We organize regular NGS data analysis courses for both academy and industry participants covering all the process including data interpretation.

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Environmental Geochemistry Group (EGG)

We can offer our experience, knowledge and advanced analytical technique to suggest solutions to current problems in actual environmental geochemistry.

  • Improvement of analytical techniques in geosciences, testing certified reference materials (CRMs)

  • Understanding the mechanisms of release of metals and metalloids from waste materials from mining and smelting

  • Suggestions of possible remediation of contaminated environment

  • Application of Raman spectrometry techniques in organic geochemistry

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Group of Coordination and Bioinorganic Chemistry

Research of the Group of Coordination and Bioinorganic Chemistry is focused on development of new medicinal probes for improved medical imaging techniques and new metal radioisotope carriers for imaging or radiotherapy. Profound expertise in fields of coordination chemistry, chemistry of macrocyclic ligands, organophosphorus chemistry, and probes for molecular imaging or therapy is offered, including:

  • Synthesis and characterization of new macrocyclic ligands and their complexes

  • Contrast agents for Magnetic Resonance Imaging (MRI)

  • Carriers of metal radioisotopes for radiodiagnostics (SPECT, PET)

  • Radiopharmaceuticals for imaging or therapy of tumors and metastases

Medicinal application of synthesized macrocyclic ligands and metal complexes as well as of their conjugates (e.g. with bis(phosphonate) group for bone targeting) is pursued. The group is interested in establishing cooperation in the fields of molecular imaging (MRI, PET, SPECT, and optical methods), radiotherapy and other applications of metal complexes in biomedical science.

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Kostrouch LAB

We offer:

  • Detection and Research of hormonally active substances in the diet and in the environment.

  • Preparation of artifi cial proteins with special properties.

  • C. elegans – genetics, genomics, genome editing

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Laboratory for Evolutionary and Ecological Immunology

The aim of studies in the Laboratory for Evolutionary and Ecological immunity is to conduct outstanding research focused on the mapping of animal interspecific and intraspecific genetic variability responsible for variation in disease resistance, and the investigation of the association between genotype and environment in forming the immunophenotype in health and disease. Since any knowledge in this field should lead to improvements in biomedicine and biotechnology, our goal is also to support the transfer of the results of our basic research into applied practice in agriculture and nature protection. Research equipment we use on daily basis includes sequencing, cloning, bioinformatics, cell biology and hematology using also flow cytometry, functional immunology testing in vivo and in vitro etc.

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Laboratory of Biomolecule Structure and Function

Laboratory of Biomolecule Structure and Function is mainly studying structure and function relationship of various important biomacromolecules (including clinically relevant ones), including target biomolecule identification, isolation and basic properties characterization, structural determination biophysical functional characterization (e.g. measurements of binding affinity), and biotechnologies leading to recombinant proteins usable as therapeutic agents. Research is mainly oriented towards proteins participating in cancer recognition, xenobiotic biotransformation, regulation of cell processes, biocatalysis, and immunomodulation in their native conditions. We offer consultancy or collaboration within the fields of:

  • Recombinant protein expression and purification in bacterial and mammalian systems, determination of disulfide bridges, glycosylation and other post-translational modifications

  • Structural biochemistry and biophysical characterization of proteins, protein-protein and protein-ligand/substrate interactions, protein crystallography

  • Structural determination and targeting by advanced techniques of mass spectrometry (photo-initiated and chemical cross-linking, H/D exchange)

  • Homology modeling, in silico ligand docking, molecular dynamics and interaction energy calculations

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Laboratory of Cellular Regenerative Medicine

We offer:

  • Isolation and cultivation of primary cells from various tissues.

  • Cultivation of commercial available cell lines.

  • Cell viability measurement.

  • Cytotoxicity measurement.

  • Immunogenicity measurement.

  • Evaluation of material biocompatibility.

  • Evaluation of cell adhesion and migration on bio-materials.

  • Fluorescent and confocal microscopy, including real-time monitoring.

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Laboratory of Immunoregulation

The aim of the research of the Laboratory of Immunoregulation is to characterize the molecular and cellular mechanisms of specific immunity which can be consequently applied in targeted immunoregulation for both the experimental work and the clinical practice. Using well established methods of laboratory routines (such as cell cultures, isolation and differentiation of stem cells, functional immunological testing in vivo and in vitro, standard immunology and cell biology assays), we are focused mainly on:

  • Study of cellular and molecular mechanisms specific to transplantation immunity

  • The specific modulation of immune responses with the aim of applying apply acquired knowledge in clinical practice

  • The use of nanofiber scaffolds as cell or drug carriers in in vitro or in vivo experimental systems

  • Investigation of the therapeutic effect of mesenchymal stem cells in experimental mouse models; study of interaction of mesenchymal stem cells with specific drugs

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Laboratory of Pharmacogenomics

We offer:

  • Research in pharmacogenetics and genomics from the clinical and academic point of view.

  • Isolation of nucleic acids from different types of tissues and cells, especially from clinical specimens.

  • Detection of single nucleotide polymorphisms and mutations by allelic discrimination, HRM, and sequen- cing.

  • Quantitative PCR in real-time (qPCR) design and gene expression analysis.

  • Help with interpretation of results of studies on metabolism and transport of anticancer drugs.

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Phycology Research Group

Phycology Research Group is focused mainly on the research of biodiversity, phylogeny, ecology, ecophysiology and symbiotic relationships of algae in lichens, investigating mostly green algae (Chlorophyta), diatoms (Bacillariophyta), freshwater conjugate algae (Zygnematophyta) and golden algae (Chrysophyta). The methods used in studies ranges from different cultivation approaches (including isolation of algae from various types of environment with further characterization of their growth features), methods of molecular genetics, bioinformatics, morphometrics, advanced microscopic tools (optical, confocal, electron microscopy) and advanced statistics.

Phycology Research Group is searching for cooperation in both fields – academic as well as in the applied research, where they can contribute mainly with their know-how in biomonitoring and the water quality assessment, waste water treatment, searching for new potential production species and strains as an alternative source for nutraceuticals, pharmaceuticals, biofuel production. Last but not least they already worked on the applied research for development of suitable coating protection of boats against the growth of algae on their surface.

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Restoration Ecology

Our mission is to try to understand the functioning  of  the  terrestrial  ecosystem  by  linking  data  about  energy   flow,  nutrient  cycling  and  biodiversity  with  a  strong  emphasis  on  the  role  of  aboveground  and  belowground  interactions.  We  then  use  that  knowledge in the restoration of ecosystems heavily  disturbed or degraded by human activity.

We can offer our expertise in the eld of reclama- tion technologies. We have great experience in post- mining site restoration as well as in the restoration of other types of habitats.

We prefer complex task that allow as to elaborate more principal research questions on side.

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Smetana LAB

We offer:

  • Cell cultivation.

  • Detection of molecules using histochemical methods.

  • Functional evaluation of cells.

  • Tissue engineering.

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Stopka LAB

We offer:

  • Cell cultivation.

  • Detection of molecules using histochemical methods.

  • Functional evaluation of cells.

  • Tissue engineering.

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Yeast Colony Group

Research of processes and mechanisms involved in the formation, development, differentiation and aging of yeast colonies and biofilms is useful as a model for simple multicellular organisms helping to elucidate functional regulations of (de)differentiating cells in colonies resembling the mechanisms in solid tumors of mammals formation (including the cancer research). Yeast biofilms can be used for testing of the effect of different conditions in the environment attacking the populations, involving drug treatments.

Development of new approaches in the study of multicellular structures of yeast colonies uses the state of art methods of genetics, molecular biology and biochemistry, e.g. for yeast genetic modification, construction of knock-outs or genomic fusions with gene tags, DNA, RNA and protein analysis, microarrays, amino acid quantification (HPLC), cell staining, flow cytometry, advanced methods for multicellular populations studies including two-photon confocal microscopy and in situ analysis of population architecture and differentiation, cell micromanipulation and separation etc. 

We offer our expertise related to molecular and cellular biology of yeast, with an emphasis on investigation and environmental resistance of multicellular populations such as biofilms, populations of natural yeast strains and immobilized populations.

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Poslední změna: 30. listopad 2018 14:30 
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Váš názor

Centrum pro přenos poznatků a technologií Univerzita Karlova

Adresa kanceláře:

Petrská 1180/3

110 00 Praha 1

Telefon: 224 491 255

Korespondenční adresa:

Ovocný trh 560/5

116 36 Praha 1

IČ: 00216208

DIČ: CZ00216208

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